猪流行性腹泻病毒S、N和ORF3基因的遗传变异分析

doi:10.11843/j.issn.0366-6964.2014.11.013

Abstract

Abstract:

To investigate the variation in structure protein of porcine epidemic diarrhea virus (PEDV)，S，N and ORF3 genes of 7 PEDV strains were cloned and sequenced from different farms in Fujian in 2012-2013. The results indicated that the nucleotide sequences of S gene shared 98.6%-99.4% identity within them，shared 93.7%-94.1% identity with attenuated DR13 strain，shared 93.8%-94.1% identity with CV777 strain. Compared with CV777 strain，there were 64 variant amino acids，and 3 amino acids different from other prevalent strains at home and abroad，and might be new variations，and 5 amino acids insertion，and 2 amino acids deletion. The amino acids insertion and deletion were similar to NPPED2008_2 strain isolated in Thailand. The nucleotide sequence of N and ORF3 genes shared 96.5%-99.7% and 99.7%-100% identity within them，and shared 96.6%-98.0% and 96.6%-96.9% identity with CV777 strain，respectively. Phylogenetic analysis indicated that S and ORF3 genes of the 7 PEDV strains had a closer relationship with the Thailand strain，and N gene had a closer relationship with the CV777 strain. The results showed that S and ORF3 genes of the 7 PEDV strains varied greatly，and these strains might origin from Thailand and Korea strains.

CLC Number:

S852.659.6

WANG Long-bai，LIN Yu-sheng，CHE Yong-liang，WU Xue-min，CHEN Ru-jing，SHAO Liang-ping，ZHOU Lun-jiang. Genetic Variation Analysis of S，N and ORF3 Genes of Porcine Epidemic Diarrhea Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1830-1836.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.11.013

https://www.xmsyxb.com/EN/Y2014/V45/I11/1830

References

［1］张强敏，郭福生,尹燕博，等.猪流行性腹泻病毒分子生物学特征［J］.中国病毒学报，2002，17(4):381-384.
［2］PENSAERT M B，DE BOUCK P. A new coronavirus-like particle associated with diarrhea in swine ［J］. Arch Virol，1978，58(3)：243-247.
［3］李佑民，柳桂信，朱维正，等.吉林省“猪传染性胃肠炎”疫情调查、人工感染传代和病毒分离试验［J］.家畜传染病，1981（1）：1-7.
［4］SÁNCHEZ C M，IZETA A，SÁNCHEZ-MORGADO J M，et al. Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropismand virulence［J］. J Virol，1999，73(9)：7607-7618.
［5］LEE D K，PARK C K，KIM S H，et al. Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea［J］. Virus Res，2010，149(2)：175-182.
［6］SONG D S，YANG J S，OH J S，et al. Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF3［J］. Vaccine，2003，21(17-18):1833-1842.
［7］KOCHERHANS R，BRIDGEN A，ACKERMANN M，et al. Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence［J］. Virus Genes，2001，23(2):137-144.
［8］YEO S G，HERNANDEZ M，KRELL P J，et al. Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99［J］.Virus Genes，2003，26(3):239-246.
［9］PARK S J，MOON H J，YANG J S，et al. Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea ［J］. Virus Genes，2007，35(2)：321-332.
［10］李建强，柳纪省，兰喜，等.猪流行性腹泻病毒纤突蛋白基因克隆与序列分析［J］.农业生物技术学报，2007，15(4):562-556.
［11］孙东波,冯力,时洪艳,等.猪流行性腹泻病毒S蛋白中和表位区单克隆抗体的制备与鉴定［J］.中国预防兽医学报，2007，29(11):887-890.
［12］刘孝珍，陈建飞，时洪艳，等.2011年猪流行性腹泻病毒的遗传变异分析［J］.中国预防兽医学报，2012，34(3):180-183.

[1]	SONG Wenyan, ZHANG Hanwen, WU Aodi, ZHANG Liyan, LIU Zhao, YE Tongtong, CHEN Chuangfu, SHENG Jinliang. Screening of Nanobodies against Porcine Reproductive and Respiratory Syndrome Virus GP5 Protein and Exploration of Their Inhibitory Effect on Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 258-270.
[2]	WANG Ying, LI Jiakang, ZENG Yue, SHI Kaituo, WEI Liting, PENG Jiajia, LI Qiuyan, CAO Longlong, ZHOU Dengyuan. Development of Monoclonal Antibodies against Feline Calicivirus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 401-405.
[3]	LI Huihui, DONG Keer, LIU Xinbo, ZHANG Chunxiao, MA Chao, CHEN Liping, ZHONG Qi, YAO Gang, MA Xuelian. Establishment and Application of Rapid Detection Method for Bovine Norovirus and Bovine Rotavirus Dual RAA-LFD [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 406-412.
[4]	ZHOU Jianhao, WANG Dongfang, LIU Ying, WANG Shujuan, MA Zhenyuan, XIE Caihua, ZHAO Xueli, YANG Haibo, FENG Guidan, KANG Taisheng, HU Yufeng, LI Bowen, YAN Ruoqian. Establishment and Preliminary Application of a Quantitative Droplet Digital PCR Assay for Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 413-418.
[5]	ZHANG Jiaqi, JIA Xining, ZHOU Qun, SONG Xin, ZHU Chenxi, LI Gege, ZHANG Bin. Construction of BVDV-1 Virus-like Particles based on E2 Protein and Evaluation of Its Immune Efficacy in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5143-5153.
[6]	ZHOU Likun, DONG Xinyuan, LI Jiahui, CUI Zhiying, ZHAO Shijie, XU Jie, CHEN Jing, ZHANG Yina, XIA Ping’an. Regulatory Role of MID2 on Porcine Reproductive and Respiratory Syndrome Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4735-4744.
[7]	ZHENG Ruwen, HUANG Tao, WU Daoyi, YU Guangmei, LI Fangfang, SUI Xin, MIN Tingyu. Establishment and Preliminary Application of LAMP-LFD Detection Method for Bovine Viral Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4745-4753.
[8]	JIA Yiquan, YU Mengting, LIU Weirong, FANG Shouguo, ZHANG Songbai. Baicalin Inhibits Infectious Bronchitis Virus Replication via Promoting the Expression of Type Ⅰ Interferon [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4839-4850.
[9]	CHEN Lu, CHANG Xinyu, SHEN Jiachen, SHEN Ruiting, ZHAO Zhenhua, HOU Xiaolin. Transcriptome Sequencing and Analysis of HD11 Cells Infected by Avian Infectious Bronchitis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4860-4865.
[10]	HE Yeqing, ZUO Baijiao, LI Qinghao, SUN Juan, JIN Xin, XIA Lu, WEI Zhanyong. Histopathological Observation of Immune Organs of Piglets Infected with Transmissible Gastroenteritis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4872-4879.
[11]	CHEN Wenzhe, ZHANG Xiangle, GU Fengxing, ZHAO Zhenxiang, LI Kangli, XUE Zhaoning, ZHENG Haixue, ZHANG Xiaoli, ZHU Zixiang. Evaluation of the Antiviral Effect of Porcine GRK2 against Foot-and-mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4350-4361.
[12]	ZOU Hong, XIA Yingju, LI Ling, XU Lu, ZHAO Junjie, WANG Tuanjie, ZHANG Qianyi, SONG Zhenhui. Establishment and Preliminary Application of a Dual Real-time RT-PCR Assay for CSFV and BVDV [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4422-4427.
[13]	YUAN Li, SUN Yangyang, ZHANG Lujie, ZHANG Jie, SUN Haifeng, BAI Juan, JIANG Ping. Production and Epitope Characterization of Monoclonal Antibodies against GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3424-3434.
[14]	CAO Liyan, KONG Xiangyu, LI Xiangtong, SUO Xuepeng, DUAN Yueyue, YUAN Cong, SHI Lei, ZHANG Yu, MA Guoxiang, ZHENG Haixue, WANG Qi. Preparation and Sequence Analysis of Monoclonal Antibody against the Nucleocapsid Protein of Porcine Acute Diarrhea Syndrome Coronavirus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2487-2497.
[15]	ZHANG Senhao, WANG Xueying, CAI Limeng, XIE Weichun, KUANG Hongdi, WANG Xiaona, LI Jiaxuan, CUI Wen, JIANG Yanping, ZHOU Han, SHAN Zhifu, WANG Li, QIAO Xinyuan, LI Yijing, TANG Lijie. Establishment and Application of Rapid Diagnosis Method for RPA of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2498-2508.

Genetic Variation Analysis of S，N and ORF3 Genes of Porcine Epidemic Diarrhea Virus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments